Contains the macro- and micronutrients and vitamins as described by Rugini (1984). This medium has been developed for the micropropagation of some types of olive cultivars. In comparison with MS medium, the formulation is more enriched in nutrients like Zn, Mg, Ca and S, and has the additional vitamins d-Biotin and folic acid.
Olive has a strong apical dominance. According to Rugini, satisfactory shoot growth only has been observed by using zeatin (mixed isomer can be used, but trans-Zeatin should be preferred).
Rugini mentioned the addition of Glutamine as organic nitrogen source (up to 2.16 g/l). It has been sterile filtered and added after autoclaving, therefore Glutamine is not part of this formula.
References:
Rugini, E. 1984. In vitro propagation of some olive (Olea europaea sativa L.) cultivars with different root-ability, and medium development using analytical data from developing shoots and embryos. Scientia Horticulturae 24(2):123-134
| Item information |
Value |
| Media components: |
macro elementsmicro elementsvitamins & organics |
| GHS-Pictograms: |
07 |
| GHS-Pictogram image: |
 |
| GHS signal word: |
Warning |
| Dosage: |
4,024 g/l |
| Hazard Statements: |
H319 |
| Hazard statements written: |
Causes serious eye irritation. |
| Precautionary Statements: |
P264-P280-P305+P351+P338-P337+P571 |
| Precautionary statements written: |
Wash skin thoroughly after handling. Wear protective gloves/ eye protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/ attention. |
| Storage Temperature: |
2 - 8 °C |
| Aussehen: |
Pulver |
| Aggregatzustand: |
Fest |
| Dosierung: |
4,03 g/l |
| Lagertemperatur: |
2 - 8 °C |
Zusammensetzung
| Makronährstoffe |
| Kaliumdihydrogenphosphat |
KH2PO4
|
340,000 |
mg/l |
| Calciumchlorid |
CaCl2
|
332,160 |
mg/l |
| Kaliumchlorid |
KCl |
500,000 |
mg/l |
| Calciumnitrat |
Ca(NO3)2
|
416,920 |
mg/l |
| Ammoniumnitrat |
NH4NO3
|
412,000 |
mg/l |
| Kaliumnitrat |
KNO3
|
1100,000 |
mg/l |
| Magnesiumsulfat |
MgSO4
|
732,530 |
mg/l |
| |
|
|
|
| Mikronährstoffe |
| Kupfer(II)Sulfat Pentahydrat |
CuSO4· 5 H2O |
0,25 |
mg/l |
| Cobalt(II)Chlorid Hexahydrat |
CoCl2· 6 H2O |
0,025 |
mg/l |
| Eisenchelat |
FeNaEDTA· 3 H2O |
42,100 |
mg/l |
| Borsäure |
H3BO3
|
12,400 |
mg/l |
| Kaliumiodid |
KI |
0,830 |
mg/l |
| Mangan(II)Sulfat Monohydrat |
MnSO4 · H2O |
16,900 |
mg/l |
| Natriummolybdat Dihydrat |
Na2MoO4· 2 H2O |
0,250 |
mg/l |
| Zink Sulfat Heptahydrat |
ZnSO4· 7 H2O |
14,300 |
mg/l |
| |
|
|
|
| Vitamine |
| d-Biotin |
|
0,050 |
mg/l |
| Glycin |
|
2,000 |
mg/l |
| myo-Inositol |
|
100,000 |
mg/l |
| Folsäure |
|
0,500 |
mg/l |
| Nikotinsäure |
|
5,000 |
mg/l |
| Pyridoxin HCl |
|
0,500 |
mg/l |
| Thiamin HCl |
|
0,500 |
mg/l |
The newest safety datasheets or certificate of analysis (CoA) can get requested by using our contact form. They also can be requested during the order process or directly per mail via [email protected].
