Rugini Olive Basic Medium

Item number: MD480

Category: Rugini Olive Medium

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Contains the macro- and micronutrients and vitamins as described by Rugini (1984). This medium has been developed for the micropropagation of some types of olive cultivars. In comparison with MS medium, the formulation is more enriched in nutrients like Zn, Mg, Ca and S, and has the additional vitamins d-Biotin and folic acid.

Olive has a strong apical dominance. According to Rugini, satisfactory shoot growth only has been observed by using zeatin (mixed isomer can be used, but trans-Zeatin should be preferred).

Rugini mentioned the addition of Glutamine as organic nitrogen source (up to 2.16 g/l). It has been sterile filtered and added after autoclaving, therefore Glutamine is not part of this formula.

 

 

References:

 

 

 

Rugini, E. 1984. In vitro propagation of some olive (Olea europaea sativa L.) cultivars with different root-ability, and medium development using analytical data from developing shoots and embryos. Scientia Horticulturae 24(2):123-134


Media components: macro elements micro elements vitamins & organics
GHS-Pictograms: 07
GHS signal word: Warning
Dosage: 4,024 g/l
Hazard Statements: H315-H319-H335-H412
Precautionary Statements: P273-P280
Storage Temperature: 2 - 8 °C

Achtung!

GHS symbol 07 Exclamation mark
H315: Verursacht Hautreizungen
H319: Verursacht schwere Augenreizung.
H335: Kann die Atemwege reizen.
H412: Schädlich für Wasserorganismen, mit langfristiger Wirkung.
   
P273: Freisetzung in die Umwelt vermeiden.
P280: Schutzhandschuhe / Augenschutz / Gesichtsschutz tragen.
Aussehen: Pulver
Aggregatzustand: Fest
Dosierung: 4,03 g/l
Lagertemperatur: 2 - 8 °C

 

 

Zusammensetzung

 

Makronährstoffe
Kaliumdihydrogenphosphat KH2PO4 340,000 mg/l
Calciumchlorid CaCl2 332,160 mg/l
Kaliumchlorid KCl 500,000 mg/l
Calciumnitrat Ca(NO3)2 416,920 mg/l
Ammoniumnitrat NH4NO3 412,000 mg/l
Kaliumnitrat KNO3 1100,000 mg/l
Magnesiumsulfat MgSO4 732,530 mg/l
       
Mikronährstoffe
Kupfer(II)Sulfat Pentahydrat CuSO4· 5 H2O 0,25 mg/l
Cobalt(II)Chlorid Hexahydrat CoCl2· 6 H2O 0,025 mg/l
Eisenchelat FeNaEDTA· 3 H2O 42,100 mg/l
Borsäure H3BO3 12,400 mg/l
Kaliumiodid KI 0,830 mg/l
Mangan(II)Sulfat Monohydrat MnSO4 · H2O 16,900 mg/l
Natriummolybdat Dihydrat Na2MoO4· 2 H2O 0,250 mg/l
Zink Sulfat Heptahydrat ZnSO4· 7 H2O 14,300 mg/l
       
Vitamine
d-Biotin   0,050 mg/l
Glycin   2,000 mg/l
myo-Inositol   100,000 mg/l
Folsäure   0,500 mg/l
Nikotinsäure   5,000 mg/l
Pyridoxin HCl   0,500 mg/l
Thiamin HCl   0,500 mg/l

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