Modification of the popular medium formulation according to Murashige & Skoog (1962) with Fe-HBED instead of Fe-EDTA. Fe-HBED has a much higher complexation constant or logK (Ma et al. 1994). Thus, it is much more stable than Fe-EDTA, as well in media with higher pH as under strong light intensity.
The usage of high stability Fe-chelates can, especially over longer cultivation stages, promote extended growth and biomass accumulation without re-dosing of Fe which gets unavailable over time due to complex destruction and formation of insoluble precipitates (Kean et al. 2015). Certain plants, however, require iron chelates with lower logK at the beginning of culture, as the uptake rate may be higher. Therefore, the sole use of Fe-HBED may lead to an initial growth-lag or even long-term performance, depending on the plant species and their individual ferrous transport system / iron uptake strategy, respectively. Mixtures of high and low logK chelates may overcome this issue: This modification can be used together with the original formula and mixed to achieve any desired ratio between Fe-HBED and Fe-EDTA.
| Item information |
Value |
| Media components: |
macro elementsmicro elementsvitamins & organics |
| GHS-Pictograms: |
07 |
| GHS-Pictogram image: |
 |
| GHS signal word: |
Warning |
| Dosage: |
4.459 g/l |
| Hazard Statements: |
H319 |
| Hazard statements written: |
Causes serious eye irritation. |
| Precautionary Statements: |
P264-P280-P305+P351+P338-P337+P559 |
| Precautionary statements written: |
Wash skin thoroughly after handling. Wear protective gloves/ eye protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/ attention. |
| References: |
Kean, M. A., Delgado, E. B., Mensink, B. P., & Bugter, M. H. J. "Iron chelating agents and their effects on the growth of Pseudokirchneriella subcapitata, Chlorella vulgaris, Phaeodactylum tricornutum and Spirulina platensis in comparison to Fe-EDTA." Journal of Algal Biomass Utilization 6.1 (2015): 56-73.
Ma, Rong, Ramunas J. Motekaitis, and Arthur E. Martell. "Stability of metal ion complexes of N, N′-bis (2-hydroxybenzyl) ethylenediamine-N, N′-diacetic acid." Inorganica chimica acta 224.1-2 (1994): 151-155.
Murashige, Toshio, and Folke Skoog. "A revised medium for rapid growth and bio assays with tobacco tissue cultures." Physiologia plantarum 15.3 (1962): 473-497.
|
| Storage Temperature: |
2 - 8 °C |
| Aussehen: |
Pulver |
| Aggregatzustand: |
Fest |
| Lagertemperatur: |
2 - 8 °C |
Zusammensetzung
| Makronährstoffe |
| Kaliumdihydrogenphosphat |
KH2PO4
|
170,000 |
mg/l |
| Calciumchlorid |
CaCl2
|
332,200 |
mg/l |
| Ammoniumnitrat |
NH4NO3
|
1650,000 |
mg/l |
| Kaliumnitrat |
KNO3
|
1900,000 |
mg/l |
| Magnesiumsulfat |
MgSO4
|
180,540 |
mg/l |
| |
|
|
|
| Mikronährstoffe |
| Kupfer(II)Sulfat Pentahydrat |
CuSO4· 5 H2O |
0,025 |
mg/l |
| Cobalt(II)Chlorid Hexahydrat |
CoCl2· 6 H2O |
0,025 |
mg/l |
| Eisenchelat |
Fe-HBED |
93,075 |
mg/l |
| Borsäure |
H3BO3
|
6,200 |
mg/l |
| Kaliumiodid |
KI |
0,830 |
mg/l |
| Mangan(II)Sulfat Monohydrat |
MnSO4 · H2O |
16,900 |
mg/l |
| Natriummolybdat Dihydrat |
Na2MoO4· 2 H2O |
0,250 |
mg/l |
| Zinksulfat Heptahydrat |
ZnSO4· 7 H2O |
8,600 |
mg/l |
| |
|
|
|
| Vitamine |
| Glycin |
|
2,000 |
mg/l |
| myo-Inositol |
|
100,000 |
mg/l |
| Nikotinsäure |
|
0,500 |
mg/l |
| Pyridoxin HCl |
|
0,500 |
mg/l |
| Thiamin HCl |
|
0,100 |
mg/l |
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