Murashige & Skoog Basic-Medium Fe-HBED mod.

Modification of the popular medium formulation according to Murashige & Skoog (1962) with Fe-HBED instead of Fe-EDTA. Fe-HBED has a much higher complexation constant or logK (Ma et al. 1994). Thus, it is much more stable than Fe-EDTA, as well in media with higher pH as under strong light intensity.

The usage of high stability Fe-chelates can, especially over longer cultivation stages, promote extended growth and biomass accumulation without re-dosing of Fe which gets unavailable over time due to complex destruction and formation of insoluble precipitates (Kean et al. 2015). Certain plants, however, require iron chelates with lower logK at the beginning of culture, as the uptake rate may be higher. Therefore, the sole use of Fe-HBED may lead to an initial growth-lag or even long-term performance, depending on the plant species and their individual ferrous transport system / iron uptake strategy, respectively. Mixtures of high and low logK chelates may overcome this issue: This modification can be used together with the original formula and mixed to achieve any desired ratio between Fe-HBED and Fe-EDTA.

Media components:	macro elements micro elements vitamins & organics
GHS-Pictograms:	07
GHS signal word:	Warning
Dosage:	4.459 g/l
Hazard Statements:	H315-H319-H335-H412
Precautionary Statements:	P273-P280
References:	Kean, M. A., Delgado, E. B., Mensink, B. P., & Bugter, M. H. J. "Iron chelating agents and their effects on the growth of Pseudokirchneriella subcapitata, Chlorella vulgaris, Phaeodactylum tricornutum and Spirulina platensis in comparison to Fe-EDTA." Journal of Algal Biomass Utilization 6.1 (2015): 56-73. Ma, Rong, Ramunas J. Motekaitis, and Arthur E. Martell. "Stability of metal ion complexes of N, N′-bis (2-hydroxybenzyl) ethylenediamine-N, N′-diacetic acid." Inorganica chimica acta 224.1-2 (1994): 151-155. Murashige, Toshio, and Folke Skoog. "A revised medium for rapid growth and bio assays with tobacco tissue cultures." Physiologia plantarum 15.3 (1962): 473-497.
Storage Temperature:	2 - 8 °C

Aussehen:	Pulver
Aggregatzustand:	Fest
Lagertemperatur:	2 - 8 °C

Zusammensetzung

Makronährstoffe
Kaliumdihydrogenphosphat	KH₂PO₄	170,000	mg/l
Calciumchlorid	CaCl₂	332,200	mg/l
Ammoniumnitrat	NH₄NO₃	1650,000	mg/l
Kaliumnitrat	KNO₃	1900,000	mg/l
Magnesiumsulfat	MgSO₄	180,540	mg/l

Mikronährstoffe
Kupfer(II)Sulfat Pentahydrat	CuSO₄· 5 H₂O	0,025	mg/l
Cobalt(II)Chlorid Hexahydrat	CoCl₂· 6 H₂O	0,025	mg/l
Eisenchelat	Fe-HBED	93,075	mg/l
Borsäure	H₃BO₃	6,200	mg/l
Kaliumiodid	KI	0,830	mg/l
Mangan(II)Sulfat Monohydrat	MnSO₄· H₂O	16,900	mg/l
Natriummolybdat Dihydrat	Na₂MoO₄· 2 H₂O	0,250	mg/l
Zinksulfat Heptahydrat	ZnSO₄· 7 H₂O	8,600	mg/l

Vitamine
Glycin		2,000	mg/l
myo-Inositol		100,000	mg/l
Nikotinsäure		0,500	mg/l
Pyridoxin HCl		0,500	mg/l
Thiamin HCl		0,100	mg/l

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