Popular gel matrix agent for plant tissue culture applications. Produces a high transparent gel. It is stable over a wide pH range and compatible to many media.
Gellan requires divalent cations like Ca
2+ to form a stable gel. If media with very low Ca
2+-concentration is used (like a MS-modification with 10 % macroelement strength), the replacement of gellan with agar is preferrable.
The formation and mechanical properties of a gellan gel is dependent from the pH. An increased pH leads to reduced junction zone formations and a decrease in molecular aggregation, reducing gel rigidity, but increasing gel deformability due to higher mobility of the strands. Low pH values lead to a higher complex viscosity (Picone & Cunha 2011).
Recommended dosage: 2 g/l, depending to the desired gel strength.
| Item information |
Value |
| GHS-Pictograms: |
00 |
| CAS-Number: |
71010-52-1 |
| EG-Number: |
275-117-5 |
| Synonyms: |
Gellan gum, Low-acyl gellan. |
| References: |
Picone, C. S. F., & Cunha, R. L. (2011). Influence of pH on formation and properties of gellan gels. Carbohydrate Polymers, 84(1), 662-668. |
| Storage Temperature: |
15 - 25 °C |
| Aussehen: |
Weißes Pulver |
| Aggregatzustand: |
Fest |
| Gelstärke: |
Min. 800 g/cm2 (je nach Medium) |
| Trocknungsverlust: |
≤ 16 % |
| Transparenz: |
Min. 85 % |
| Lagerung: |
RT |
| Autoklavierbar: |
Ja |
Technische Daten
| Molare Masse (M): |
1000 kg/mol |
| pH Wert: |
7 |
| >Geliertemperatur: |
27 - 32 °C * |
| CAS-Nr.: |
71010-52-1 |
| EG-Nr.: |
275-117-5 |
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