WPM | McCown's Woody Plant Basic Medium

Item number: MD1030

Category: McCown's Woody Plant

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Contains macro and micronutrients and vitamins such as described by Lloyd & McCown (1980). It is produced by using a corrected formula according to Owen & Miller (1992) with 100 µM manganese(II)-sulfate. Thereby its concentration is adjusted to the formulation which is also called later as the popular medium "WPM" (Smith and McCown, 1983).

Please notice: Despite the name that the originators have given to this medium, it is not universally suitable for all woody plant species. This medium has originally been developed for micropropagation of Kalmia latifolia.

References:

Lloyd, G & B McCown. 1980. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Combined Proceedings, International Plant Propagators' Society 30:421-427
Owen, H. R. and Miller, A. R. (1992) ‘An examination and correction of plant tissue culture basal medium formulations’, Plant Cell, Tissue and Organ Culture.
Smith, M. A. L. and McCown, B. H. (1983) ‘A Comparison of Source Tissue for Protoplast Isolation from Three Woody Plant Species’, Plant Science Letters, 28(2), pp. 149–156.


Media components: macro elements micro elements vitamins & organics
Release class: Release class 0
GHS-Pictograms: 07
GHS signal word: Warning
Dosage: 2.374 g/l
Hazard Statements: H315-H319-H335-H412
Precautionary Statements: P273-P280
Storage Temperature: 2 - 8 °C

Achtung!

GHS symbol 07 Exclamation mark
H315: Verursacht Hautreizungen
H319: Verursacht schwere Augenreizung.
H335: Kann die Atemwege reizen.
H412: Schädlich für Wasserorganismen, mit langfristiger Wirkung.
   
P273: Freisetzung in die Umwelt vermeiden.
P280: Schutzhandschuhe / Augenschutz / Gesichtsschutz tragen.
P305+P351+P338: BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen.
Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.
Appearance: white to yellowish powder
Storage temperature: 2 - 8 °C

Formula

Macroelements
Potassium dihydrogen phosphate KH2PO4 170,000 mg/l
Calcium nitrate (anh.) Ca(NO3)2 386,330 mg/l
Calcium chloride CaCl2 72,500 mg/l
Potassium sulfate K2SO4 990,000 mg/l
Ammonium nitrate NH4NO3 400,000 mg/l
Magnesium sulfate (anh.) MgSO4 180,540 mg/l
Microelements
Copper(II) sulfate pentahydrate CuSO4· 5 H2O 0,250 mg/l
Iron-Chelate FeNaEDTA 42,110 mg/l
Boric acid H3BO3 6,200 mg/l
Manganese(II) sulfate monohydrate MnSO4 · H2O 16,900 mg/l
Sodium molybdate dihydrate Na2MoO4· 2 H2O 0,250 mg/l
Zinc sulfate heptahydrate ZnSO4· 7 H2O 8,600 mg/l
Vitamins / Organics
Glycine 2,000 mg/l
myo-Inositol 100,000 mg/l
Nicotinic acid 0,500 mg/l
Pyridoxine HCl 0,500 mg/l
Thiamine HCl 1,000 mg/l

The newest safety datasheets or certificate of analysis (CoA) can get requested by using our contact form. They also can be requested during the order process or directly per mail via [email protected].