Contains macro and micronutrients and vitamins such as described by Lloyd & McCown (1980). It is produced by using a corrected formula according to Owen & Miller (1992) with 100 µM manganese(II)-sulfate. Thereby its concentration is adjusted to the formulation which is also called later as the popular medium "WPM" (Smith and McCown, 1983).
Please notice: Despite the name that the originators have given to this medium, it is not universally suitable for all woody plant species. This medium has originally been developed for micropropagation of Kalmia latifolia.
References:
Lloyd, G & B McCown. 1980. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Combined Proceedings, International Plant Propagators' Society 30:421-427
Owen, H. R. and Miller, A. R. (1992) ‘An examination and correction of plant tissue culture basal medium formulations’, Plant Cell, Tissue and Organ Culture.
Smith, M. A. L. and McCown, B. H. (1983) ‘A Comparison of Source Tissue for Protoplast Isolation from Three Woody Plant Species’, Plant Science Letters, 28(2), pp. 149–156.
| Item information |
Value |
| Release class: |
Release class 0 |
| Media components: |
macro elementsmicro elementsvitamins & organics |
| GHS-Pictograms: |
07 |
| GHS-Pictogram image: |
 |
| GHS signal word: |
Warning |
| Dosage: |
2.374 g/l |
| Hazard Statements: |
H319 |
| Hazard statements written: |
Causes serious eye irritation. |
| Precautionary Statements: |
P264-P280-P305+P351+P338-P337+P315 |
| Precautionary statements written: |
Wash skin thoroughly after handling. Wear protective gloves/ eye protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/ attention. |
| Storage Temperature: |
2 - 8 °C |
| SVHC > 0.1 mass-%: |
Boric acid, CAS-Nr. 10043-35-3. |
| Appearance: |
white to yellowish powder |
|
|
| Storage temperature: |
2 - 8 °C |
Formula
| Macroelements |
| Potassium dihydrogen phosphate |
KH2PO4
|
170,000 |
mg/l |
| Calcium nitrate (anh.) |
Ca(NO3)2
|
386,330 |
mg/l |
| Calcium chloride |
CaCl2
|
72,500 |
mg/l |
| Potassium sulfate |
K2SO4
|
990,000 |
mg/l |
| Ammonium nitrate |
NH4NO3
|
400,000 |
mg/l |
| Magnesium sulfate (anh.) |
MgSO4
|
180,540 |
mg/l |
|
|
|
|
| Microelements |
| Copper(II) sulfate pentahydrate |
CuSO4· 5 H2O |
0,250 |
mg/l |
| Iron-Chelate |
FeNaEDTA |
42,110 |
mg/l |
| Boric acid |
H3BO3
|
6,200 |
mg/l |
| Manganese(II) sulfate monohydrate |
MnSO4 · H2O |
16,900 |
mg/l |
| Sodium molybdate dihydrate |
Na2MoO4· 2 H2O |
0,250 |
mg/l |
| Zinc sulfate heptahydrate |
ZnSO4· 7 H2O |
8,600 |
mg/l |
|
|
|
|
| Vitamins / Organics |
| Glycine |
|
2,000 |
mg/l |
| myo-Inositol |
|
100,000 |
mg/l |
| Nicotinic acid |
|
0,500 |
mg/l |
| Pyridoxine HCl |
|
0,500 |
mg/l |
| Thiamine HCl |
|
1,000 |
mg/l |
The newest safety datasheets or certificate of analysis (CoA) can get requested by using our contact form. They also can be requested during the order process or directly per mail via [email protected].
