The New Dogashima Medium (NDM) was developed for micropropagation of orchids like Phalaenopsis species (Tokuhara and Mii, 1993). Organic components are based on a modification of the Morel & Wetmore organics (Morel & Wetmore, 1951) including the addition of adenine and cysteine. The authors reported enhanced growth of protocorm like bodies (PLB) and higher survival rates of Phalaenopsis plants with NDM compared to Murashige and Skoog medium.
Please notice: This formulation contains temperature sensitive substances. Autoclaving time should not be unnecessarily long to prevent higher degradation rates. As an alternative, we also offer the separate mixture of sensitive organic reagents which can be sterilized by sterile filtration and added after autoclaving.
Dehydrated powder, dosage: 1.96 g/l
References:
Morel, G. and Wetmore, R. H. (1951). Fern Callus Tissue Culture. American Journal of Botany, 38(2):141
Tokuhara, K & M Mii. 1993. Micropropagation of Phalaenopsis and Doritaenopsis by culturing shoot tips of flower stalk buds. Plant Cell Rep, 13:7-11
| Item information |
Value |
| Media components: |
macro elementsmicro elementsvitamins & organics |
| GHS-Pictograms: |
07 |
| GHS-Pictogram image: |
 |
| GHS signal word: |
Warning |
| Dosage: |
1,96 g/l |
| Hazard Statements: |
H319 |
| Hazard statements written: |
Causes serious eye irritation. |
| Precautionary Statements: |
P264-P280-P305+P351+P338-P337+P434 |
| Precautionary statements written: |
Wash skin thoroughly after handling. Wear protective gloves/ eye protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/ attention. |
| Storage Temperature: |
2 - 8 °C |
| Aussehen: |
Pulver |
| Aggregatzustand: |
fest |
| Lagerung: |
2 - 8 °C |
Zusammensetzung
| Makronährstoffe |
| Ammoniumnitrat |
NH4NO3
|
480,000 |
mg/l |
| Calciumnitrat |
Ca(NO3)2
|
326,389 |
mg/l |
| Kaliumdihydrogenphosphat |
KH2PO4
|
550,000 |
mg/l |
| Kaliumchlorid |
KCl |
150,000 |
mg/l |
| Kaliumnitrat |
KNO3
|
200,000 |
mg/l |
| Magnesiumsulfat |
MgSO4
|
122,500 |
mg/l |
|
|
|
|
| Mikronährstoffe |
| Kupfer(II)Sulfat Pentahydrat |
CuSO4· 5 H2O |
0,025 |
mg/l |
| Cobalt(II)Chlorid Hexahydrat |
CoCl2· 6 H2O |
0,025 |
mg/l |
| Eisenchelat |
FeNaEDTA |
21,000 |
mg/l |
| Borsäure |
H3BO3
|
0,500 |
mg/l |
| Mangan(II)Sulfat Monohydrat |
MnSO4 · H2O |
3,000 |
mg/l |
| Natriummolybdat Dihydrat |
Na2MoO4· 2 H2O |
0,025 |
mg/l |
| Zinksulfat Heptahydrat |
ZnSO4· 7 H2O |
0,500 |
mg/l |
|
|
|
|
| Vitamine |
| Nicotinsäure |
|
1,000 |
mg/l |
| myo-Inositol |
|
100,000 |
mg/l |
| Calciumpanthothenat |
|
1,000 |
mg/l |
| Pyridoxin HCl |
|
1,000 |
mg/l |
| Thiamin HCl |
|
1,000 |
mg/l |
| Biotin |
|
0,100 |
mg/l |
|
|
|
|
| Aminosäuren |
| L-Cystein |
|
1,000 |
mg/l |
|
|
|
|
| Sonstige |
| Adenin-Hemisulfat |
|
1,370 |
mg/l |
|
|
|
|
The newest safety datasheets or certificate of analysis (CoA) can get requested by using our contact form. They also can be requested during the order process or directly per mail via [email protected].
