NDM | New Dogashima Medium

Item number: MD220

Category: New Dogashima


There are variations of this item. Please select the variation you wish.

The New Dogashima Medium (NDM) was developed for micropropagation of orchids like Phalaenopsis species (Tokuhara and Mii, 1993). Organic components are based on a modification of the Morel & Wetmore organics (Morel & Wetmore, 1951) including the addition of adenine and cysteine. The authors reported enhanced growth of protocorm like bodies (PLB) and higher survival rates of Phalaenopsis plants with NDM compared to Murashige and Skoog medium.

Please notice: This formulation contains temperature sensitive substances. Autoclaving time should not be unnecessarily long to prevent higher degradation rates. As an alternative, we also offer the separate mixture of sensitive organic reagents which can be sterilized by sterile filtration and added after autoclaving.

Dehydrated powder, dosage: 1.96 g/l


Morel, G. and Wetmore, R. H. (1951). Fern Callus Tissue Culture. American Journal of Botany, 38(2):141
Tokuhara, K & M Mii. 1993. Micropropagation of Phalaenopsis and Doritaenopsis by culturing shoot tips of flower stalk buds. Plant Cell Rep, 13:7-11

Media components: macro elements micro elements vitamins & organics
GHS-Pictograms: 07
Dosage: 1,96 g/l
GHS signal word: Warning
Hazard Statements: H315-H319-H335-H412
Precautionary Statements: P273-P280
Storage Temperature: 2 - 8 °C
Aussehen: Pulver
Aggregatzustand: fest
Lagerung: 2 - 8 °C


Ammoniumnitrat NH4NO3 480,000 mg/l
Calciumnitrat Ca(NO3)2 326,389 mg/l
Kaliumdihydrogenphosphat KH2PO4 550,000 mg/l
Kaliumchlorid KCl 150,000 mg/l
Kaliumnitrat KNO3 200,000 mg/l
Magnesiumsulfat MgSO4 122,500 mg/l
Kupfer(II)Sulfat Pentahydrat CuSO4· 5 H2O 0,025 mg/l
Cobalt(II)Chlorid Hexahydrat CoCl2· 6 H2O 0,025 mg/l
Eisenchelat FeNaEDTA 21,000 mg/l
Borsäure H3BO3 0,500 mg/l
Mangan(II)Sulfat Monohydrat MnSO4 · H2O 3,000 mg/l
Natriummolybdat Dihydrat Na2MoO4· 2 H2O 0,025 mg/l
Zinksulfat Heptahydrat ZnSO4· 7 H2O 0,500 mg/l
Nicotinsäure 1,000 mg/l
myo-Inositol 100,000 mg/l
Calciumpanthothenat 1,000 mg/l
Pyridoxin HCl 1,000 mg/l
Thiamin HCl 1,000 mg/l
Biotin 0,100 mg/l
L-Cystein 1,000 mg/l
Adenin-Hemisulfat 1,370 mg/l

The newest safety datasheets or certificate of analysis (CoA) can get requested by using our contact form. They also can be requested during the order process or directly per mail via [email protected].