The New Dogashima Medium (NDM) was developed for micropropagation of orchids like Phalaenopsis species (Tokuhara and Mii, 1993). This mixture only contains organic components, based on a modification of the Morel & Wetmore organics (Morel & Wetmore, 1951) including the addition of adenine and cysteine. The authors reported enhanced growth of protocorm like bodies (PLB) and higher survival rates of Phalaenopsis plants with NDM compared to Murashige and Skoog medium.
The mixture allows separate sterile filtration to protect temperature sensitive components, whereas nutrients must be supplied separately (they can be autoclaved). The powder mixture can be stored at 2 - 8 °C, but stock solutions should be stored at -20 - 0 °C.
Morel, G. and Wetmore, R. H. (1951). Fern Callus Tissue Culture. American Journal of Botany, 38(2):141
Tokuhara, K & M Mii. 1993. Micropropagation of Phalaenopsis and Doritaenopsis by culturing shoot tips of flower stalk buds. Plant Cell Rep, 13:7-11
|Media components:||vitamins & organics|
|Storage Temperature:||2 - 8 °C|
|Storage:||2 - 8 °C|
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