The New Dogashima Medium (NDM) was developed for micropropagation of orchids like Phalaenopsis species (Tokuhara and Mii, 1993). The authors reported enhanced growth of protocorm like bodies (PLB) and higher survival rates of Phalaenopsis plants with NDM compared to Murashige and Skoog medium.
This formulation only contains macro- and microelements. Vitamins and other organic substances must be added separately after autoclaving.
References:
Morel, G. and Wetmore, R. H. (1951). Fern Callus Tissue Culture. American Journal of Botany, 38(2):141
Tokuhara, K & M Mii. 1993. Micropropagation of Phalaenopsis and Doritaenopsis by culturing shoot tips of flower stalk buds. Plant Cell Rep, 13:7-11
| Item information |
Value |
| Release class: |
Release class 0 |
| Media components: |
macro elementsmicro elements |
| GHS-Pictograms: |
07 |
| GHS-Pictogram image: |
 |
| Dosage: |
1.858 g/l |
| GHS signal word: |
Warning |
| Hazard Statements: |
H319 |
| Hazard statements written: |
Causes serious eye irritation. |
| Precautionary Statements: |
P264-P280-P305+P351+P338-P337+P726 |
| Precautionary statements written: |
Wash skin thoroughly after handling. Wear protective gloves/ eye protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/ attention. |
| Storage Temperature: |
15 - 25 °C |
| Aussehen: |
Pulver |
| Aggregatzustand: |
fest |
| Lagerung: |
2 - 8 °C |
Zusammensetzung
| Makronährstoffe |
| Ammoniumnitrat |
NH4NO3
|
480,000 |
mg/l |
| Calciumnitrat |
Ca(NO3)2
|
326,389 |
mg/l |
| Kaliumdihydrogenphosphat |
KH2PO4
|
550,000 |
mg/l |
| Kaliumchlorid |
KCl |
150,000 |
mg/l |
| Kaliumnitrat |
KNO3
|
200,000 |
mg/l |
| Magnesiumsulfat |
MgSO4
|
122,500 |
mg/l |
|
|
|
|
| Mikronährstoffe |
| Kupfer(II)Sulfat Pentahydrat |
CuSO4· 5 H2O |
0,025 |
mg/l |
| Cobalt(II)Chlorid Hexahydrat |
CoCl2· 6 H2O |
0,025 |
mg/l |
| Eisenchelat |
FeNaEDTA |
21,000 |
mg/l |
| Borsäure |
H3BO3
|
0,500 |
mg/l |
| Mangan(II)Sulfat Monohydrat |
MnSO4 · H2O |
3,000 |
mg/l |
| Natriummolybdat Dihydrat |
Na2MoO4· 2 H2O |
0,025 |
mg/l |
| Zinksulfat Heptahydrat |
ZnSO4· 7 H2O |
0,500 |
mg/l |
|
|
|
|
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