The BM (Basic Medium) was developed for the germination of Western European orchids by Van Waes and Debergh (1986). After testing 23 different species, the authors concluded that European orchids are more sensitive to salts compared to tropical orchids. The germination rate was higher on less saline media. In BM1 or BM2, nitrogen was not added via inorganic sources, but via casein hydrolysate and glutamine.
BM2 is composed like BM1, but additionally contains 0.88 µM BAP. This cytokinin led to an increase in the germination rate in some species and a decrease in others. In the following list, the species with increased germination rates are assigned to the respective medium:
BM1:
Anacamptis pyramidalis
Dactylorhiza spp.
Gymnadenia spp.
Listera ovata Ophrys spp.
Orchis spp.
Platanthera spp.
Spiranthes spp.
BM2:
Aceras anthropophorum
Cephalanthera spp.
Cypripedium calceolus
Epipactis spp.
Limodorum abortivum
A purified casein hydrolysate with lowered salt and high nitrogen content is used for this medium. This is not identical to the hydrolysate used by Van Waes and Debergh. Please contact us if you require the formula with the variant originally used.
| Item information |
Value |
| Media components: |
macro elementsmicro elementsvitamins & organics |
| GHS-Pictograms: |
07 |
| GHS-Pictogram image: |
 |
| Dosage: |
1.192 g/l |
| GHS signal word: |
Warning |
| Hazard Statements: |
H319 |
| Hazard statements written: |
Causes serious eye irritation. |
| Precautionary Statements: |
P264-P280-P305+P351+P338-P337+P691 |
| Precautionary statements written: |
Wash skin thoroughly after handling. Wear protective gloves/ eye protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/ attention. |
| References: |
Van Waes, J. M.; Debergh, P. C. In vitro germination of some Western European orchids. Physiologia Plantarum, 1986, 67. Jg., Nr. 2, S. 253-261. |
| Storage Temperature: |
2 - 8 °C |
| SVHC > 0.1 mass-%: |
Boric acid, CAS-Nr. 10043-35-3. |
| Aussehen: |
Pulver |
| Aggregatzustand: |
fest |
| Lagerung: |
2 - 8 °C |
Zusammensetzung
| Makronährstoffe |
| Kaliumdihydrogenphosphat |
KH2PO4
|
300,000 |
mg/l |
| Magnesiumsulfat |
MgSO4
|
100,000 |
mg/l |
| |
|
|
|
| Mikronährstoffe |
| Kupfer(II)Sulfat Pentahydrat |
CuSO4· 5 H2O |
0,025 |
mg/l |
| Cobalt(II)Chlorid Hexahydrat |
CoCl2· 6 H2O |
0,025 |
mg/l |
| Eisenchelat |
FeNaEDTA |
36,700 |
mg/l |
| Borsäure |
H3BO3
|
10,000 |
mg/l |
| Mangan(II)Sulfat Monohydrat |
MnSO4 · H2O |
25,000 |
mg/l |
| Natriummolybdat Dihydrat |
Na2MoO4· 2 H2O |
0,250 |
mg/l |
| Zinksulfat Heptahydrat |
ZnSO4· 7 H2O |
10,000 |
mg/l |
| |
|
|
|
| Vitamine |
| Biotin |
|
0,050 |
mg/l |
| Folsäure |
|
0,500 |
mg/l |
| Glycin |
|
2,000 |
mg/l |
| myo-Inositol |
|
100,000 |
mg/l |
| Nikotinsäure |
|
5,000 |
mg/l |
| Pyridoxin HCl |
|
0,500 |
mg/l |
| Thiamin HCl |
|
0,500 |
mg/l |
| |
|
|
|
| Aminosäuren |
| L-Glutamin |
|
100,000 |
mg/l |
| |
|
|
|
| Weitere Zusätze |
| Benzylaminopurin |
|
0,200 |
mg/l |
| Casein (Hydrolysat) |
|
500,000 |
mg/l |
The newest safety datasheets or certificate of analysis (CoA) can get requested by using our contact form. They also can be requested during the order process or directly per mail via [email protected].
