Nitsch & Nitsch originally developed this medium for the cultivation of haploid Nicotiana microspores. This medium is now widely used for the culture of haploid plants of different species. It contains macro- and micronutrients and vitamins such as described by Nitsch & Nitsch (1969).
Powder, dissolve in water. Dosage: 2.18 g/l
References:
Nitsch, JP & C Nitsch. 1969. Haploid plants from pollen grains. Science 163(3862):85-87
| Item information |
Value |
| Release class: |
Release class 0 |
| Media components: |
macro elementsmicro elementsvitamins & organics |
| GHS-Pictograms: |
07 |
| GHS-Pictogram image: |
 |
| GHS signal word: |
Warning |
| Dosage: |
2.18 g/l |
| Hazard Statements: |
H319 |
| Hazard statements written: |
Causes serious eye irritation. |
| Precautionary Statements: |
P264-P280-P305+P351+P338-P337+P324 |
| Precautionary statements written: |
Wash skin thoroughly after handling. Wear protective gloves/ eye protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/ attention. |
| Storage Temperature: |
2 - 8 °C |
| SVHC > 0.1 mass-%: |
Boric acid, CAS-Nr. 10043-35-3. |
| Aussehen: |
Pulver |
| Aggregatzustand: |
fest |
| Lagerung: |
2 - 8 °C |
Zusammensetzung
| Makronährstoffe |
| Kaliumdihydrogenphosphat |
KH2PO4
|
68,000 |
mg/l |
| Calciumchlorid |
CaCl2
|
166,000 |
mg/l |
| Ammoniumnitrat |
NH4NO3
|
720,000 |
mg/l |
| Kaliumnitrat |
KNO3
|
950,000 |
mg/l |
| Magnesiumsulfat |
MgSO4
|
90,270 |
mg/l |
|
|
|
|
| Mikronährstoffe |
| Kupfer(II)Sulfat Pentahydrat |
CuSO4· 5 H2O |
0,025 |
mg/l |
| Eisenchelat |
FeNaEDTA |
36,700 |
mg/l |
| Borsäure |
H3BO3
|
6,200 |
mg/l |
| Mangan(II)Sulfat Monohydrat |
MnSO4 · H2O |
18,940 |
mg/l |
| Natriummolybdat Dihydrat |
Na2MoO4· 2 H2O |
0,250 |
mg/l |
| Zinksulfat Heptahydrat |
ZnSO4· 7 H2O |
10,000 |
mg/l |
|
|
|
|
| Vitamine |
| Biotin |
|
0,050 |
mg/l |
| Folsäure |
|
0,500 |
mg/l |
| Glycin |
|
2,000 |
mg/l |
| myo-Inositol |
|
100,000 |
mg/l |
| Nikotinsäure |
|
5,000 |
mg/l |
| Pyridoxin HCl |
|
0,500 |
mg/l |
| Thiamin HCl |
|
0,500 |
mg/l |
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