Murashige & Skoog Basic Medium, MES-buffered

Item number: MD073

Category: Basic Medium MES buffered

Quantity

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Also known as MS-Medium. Contains macroelements, microelements and vitamins such as described by MURASHIGE & SKOOG (1962). Contains MES-Buffer. This buffer is not metabolized by eucaryotic cells. Buffering range: pH 5,5 - 6,7

Warning: Autoclaving of MES buffer, especially in combination with MS salts, is not recommended. A more efficient way is to add sterile filtrated MES buffer after autoclaving. However, this formulation can be used to test quickly if buffering solves problems like pH drop during culture. Please don't try to filtrate concentrated stock solutions, it will block the filter due to formation of precipitates immediately.

References:

Murashige, T & F. Skoog. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum 15(3):473-497


Media components: macro elements micro elements vitamins & organics additives
Release class: Release class 0
GHS-Pictograms: 07
GHS signal word: Warning
Dosage: 4,905 g/l
Hazard Statements: H315-H319-H335-H412
Precautionary Statements: P273-P280
Storage Temperature: 2 - 8 °C

Achtung!

GHS symbol 07 Exclamation mark
H315: Verursacht Hautreizungen
H319: Verursacht schwere Augenreizung.
H335: Kann die Atemwege reizen.
H412: Schädlich für Wasserorganismen, mit langfristiger Wirkung.
   
P273: Freisetzung in die Umwelt vermeiden.
P280: Schutzhandschuhe / Augenschutz / Gesichtsschutz tragen.
P305+P351+P338: BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen.
Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.
Aussehen: Pulver
Aggregatzustand: fest
Lagerung: 2 - 8 °C

Zusammensetzung

Makronährstoffe
Kaliumdihydrogenphosphat KH2PO4 170,000 mg/l
Calciumchlorid CaCl2 332,200 mg/l
Ammoniumnitrat NH4NO3 1650,000 mg/l
Kaliumnitrat KNO3 1900,000 mg/l
Magnesiumsulfat MgSO4 180,540 mg/l
Mikronährstoffe
Kupfer(II)Sulfat Pentahydrat CuSO4· 5 H2O 0,025 mg/l
Cobalt(II)Chlorid Hexahydrat CoCl2· 6 H2O 0,025 mg/l
Eisenchelat FeNaEDTA 36,700 mg/l
Borsäure H3BO3 6,200 mg/l
Kaliumiodid KI 0,830 mg/l
Mangan(II)Sulfat Monohydrat MnSO4 · H2O 16,900 mg/l
Natriummolybdat Dihydrat Na2MoO4· 2 H2O 0,250 mg/l
Zinksulfat Heptahydrat ZnSO4· 7 H2O 8,600 mg/l
Vitamine
Glycin 2,000 mg/l
myo-Inositol 100,000 mg/l
Nikotinsäure 0,500 mg/l
Pyridoxin HCl 0,500 mg/l
Thiamin HCl 0,100 mg/l
Sonstige
MES Monohydrat 500,000 mg/l

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