Requirements for a PTC-Lab
A plant tissue culture laboratory has several requirements to enable a successful work. A short overview:
Washing and storage of glass-, plastic- and other labwares can not be avoided and should be performed in a way which allows to provide clean conditions. For a commercial purposes, an industrial dish washer is desirable. Contaminated culture vials should be autoclaved before washing or discarding to minimize spreading of bacterial and fungal contaminants.
The preparation of plant tissue culture media requires capabilities for sterilization and proper storage of reagents and stock solutions. The preparation is a critical step in tissue culture work in which great precision is required.
Tap water is not recommended for medium preparation, as it contains impurities such as inorganic and organic compounds and microorganisms. Suitable water quality can be obtained through distillation, deionization or reverse osmosis. Depending on the available water quality, a combination of those methods is required.
Sterile working area
Enables aseptic handling of plant material. The best environment can be provided through a laminar flow hood, which are commercially available. A DIY workbench can save costs for smaller private / hobby PTC labs.
The maintenance of cultures is optimal under controlled conditions of temperature, light and humidity. Environmental chambers support the reproducibility of protocols and protect sensitive cultures by retaining the control over physical parameters.
The inspection of cultures is critical and should be performed in regular intervals. It helps to keep the survival capacity at high rates.