innuPREP Plant DNA Extraction Kit

Item number: EQ110

Category: Kits


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Shipping time: 1 - 3 workdays

47,63 $ *

Shipping time: 1 - 3 workdays

189,21 $ *

Shipping time: 1 - 3 workdays

748,91 $ *
The innuPREP Plant DNA Kit has been specially developed for quickly and easily isolating DNA from an extremely wide variety of plant starting materials (such as leaves, stems, roots, flowers, wood, seeds, fruits etc.). The novel Three-Lysis Buffer System (based on e.g. CTAB or SDS lysis) allows perfect adaption to an kind of plant and their constituent parts. In addition to efficient sample lysis, the extraction routine also includes a prefiltration step to effectively minimize unlysed plant components and to clarify the lysate. The DNA is then bound to a Spin Filter column using an optimized Binding Buffer, after which it is washed and then eluted in a separate Elution Tube. The extracted nucleic acid is then immediately available for a number of downstream applications and can be stored for future use without any trouble.

Product features

  • Isolation of highly pure DNA free of plant inhibitors and secondary metabolic products
  • High yields from up to 180 mg starting material
  • Get rid of specialized kits: Three-Lysis Buffer System for ideal adaption to different plants and their constituent parts
  • Specific guidelines and recommendations for homogenization according to kind of sample
  • Tested for fresh or frozen plant sample (including dry archived material)
  • Perfect preparation conditions for leafs, wood, seeds, needles, fruits and much more
  • Including prefilters for clearing the lysate


1. Homogenize and lyse the plant material; follow with a prefiltration step (use Prefilter)
2. Bind the DNA to the Spin Filter
3. Wash the bound DNA
4. Elute the DNA


MPG Data  
CE IVD No
Manufacturer AJ Innuscreen GmbH


Extraction

 
A260 : A280 1.7 - 2.0
Starting material for extraction

- Various plant materials e.g. wood, seed, needles, fruits etc.
- Up to 100 mg dry weight or up to 180 mg wet weigth
- Fresh, frozen or dried plant samples

Ų Yield

- Depends on the type and starting quantity of the plant material
- Approx. 3 - 25 µg

Binding capacity > 50 µg genomic DNA
Elution volume 1 - 2times of 100 - 200 µl Elution Buffer
Duration of extraction Approx. 30 to 40 minutes
Basis Filter membrane
Handling Manual processing of Spin Filter columns
Storage / Stability of extraction kit    12 month at room temperature (14 °C - 25 °C)



Recommendation
Instruments for automatized DNA extraction and downstream applications like PCR-Thermocyclers can be found at
Analytik Jena | Section Life Science

Storage Temperature: 15 - 25 °C
Storage notice: The innuPREP Plant DNA Kit will remain stable for at least 12 months if stored in a dry place at room temperature (14 °C to 25 °C). The recommended storage temperature for lyophilized Proteinase K is 4 °C. Once the Proteinase K has been solubilized, it should be stored in aliquots at -20 °C, because repeated freezing and thawing will significantly reduce its activity.
Items delivered:
  • Lysis Solution
  • Binding Solution
  • Precipitation Buffer P
  • Washing Solutions
  • Elution Buffer
  • Prefilter (lavender)
  • Spin Filter (green)
  • Receiver Tubes
  • Elution Tubes
  • user manual

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