Terrestrial Orchid Medium

The BM (Basic Medium) was developed for the germination of Western European orchids by Van Waes and Debergh (1986). After testing 23 different species, the authors concluded that European orchids are more sensitive to salts compared to tropical orchids. The germination rate was higher on less saline media. In BM1 or BM2, nitrogen was not added via inorganic sources, but via casein hydrolysate and glutamine.

BM2 is composed like BM1, but additionally contains 0.88 µM BAP. This cytokinin led to an increase in the germination rate in some species and a decrease in others. In the following list, the species with increased germination rates are assigned to the respective medium:

BM1:

Anacamptis pyramidalis
Dactylorhiza spp.
Gymnadenia spp.
Listera ovata Ophrys spp.
Orchis spp.
Platanthera spp.
Spiranthes spp.

BM2:

Aceras anthropophorum
Cephalanthera spp.
Cypripedium calceolus
Epipactis spp.
Limodorum abortivum

A purified casein hydrolysate with lowered salt and high nitrogen content is used for this medium. This is not identical to the hydrolysate used by Van Waes and Debergh. Please contact us if you require the formula with the variant originally used.

Media components:	macro elements micro elements vitamins & organics carbon source
Release class:	Release class 0
GHS-Pictograms:	00
Dosage:	21.191 g/l
References:	Van Waes, J. M.; Debergh, P. C. In vitro germination of some Western European orchids. Physiologia Plantarum, 1986, 67. Jg., Nr. 2, S. 253-261.
Storage Temperature:	2 - 8 °C

Aussehen:	Pulver
Aggregatzustand:	fest
Lagerung:	2 - 8 °C

Zusammensetzung

Makronährstoffe
Kaliumdihydrogenphosphat	KH₂PO₄	300,000	mg/l
Magnesiumsulfat	MgSO₄	100,000	mg/l

Mikronährstoffe
Kupfer(II)Sulfat Pentahydrat	CuSO₄· 5 H₂O	0,025	mg/l
Cobalt(II)Chlorid Hexahydrat	CoCl₂· 6 H₂O	0,025	mg/l
Eisenchelat	FeNaEDTA	36,700	mg/l
Borsäure	H₃BO₃	10,000	mg/l
Mangan(II)Sulfat Monohydrat	MnSO₄· H₂O	25,000	mg/l
Natriummolybdat Dihydrat	Na₂MoO₄· 2 H₂O	0,250	mg/l
Zinksulfat Heptahydrat	ZnSO₄· 7 H₂O	10,000	mg/l

Vitamine
Biotin		0,050	mg/l
Folsäure		0,500	mg/l
Glycin		2,000	mg/l
myo-Inositol		100,000	mg/l
Nikotinsäure		5,000	mg/l
Pyridoxin HCl		0,500	mg/l
Thiamin HCl		0,500	mg/l

Aminosäuren
L-Glutamin		100,000	mg/l

Weitere Zusätze
Casein (Hydrolysat)		500,000	mg/l
Saccharose		20000,000	mg/l

The newest safety datasheets or certificate of analysis (CoA) can get requested by using our contact form. They also can be requested during the order process or directly per mail via [email protected].

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BM2 | Terrestrial Orchid Medium incl. Sucrose

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Murashige & Skoog Basic Medium incl. Vitamins

BM1 | Terrestrial Orchid Medium incl. Sucrose