Nitsch & Nitsch Basic Medium
Nitsch & Nitsch originally developed this medium for the cultivation of haploid Nicotiana microspores. This medium is now widely used for the culture of haploid plants of different species. It contains macro- and micronutrients and vitamins such as described by Nitsch & Nitsch (1969).
Powder, dissolve in water. Dosage: 2.18 g/l
References:
Nitsch, JP & C Nitsch. 1969. Haploid plants from pollen grains. Science 163(3862):85-87
| GHS-Pictograms: | 07 |
| GHS signal word: | Warning |
| Dosage: | 2.18 g/l |
| Hazard Statements: | H315-H319-H335-H412 |
| Precautionary Statements: | P273-P280 |
| Storage Temperature: | 2 - 8 °C |
Achtung! |
|
|
|
| H315: | Verursacht Hautreizungen |
| H319: | Verursacht schwere Augenreizung. |
| H335: | Kann die Atemwege reizen. |
| H412: | Schädlich für Wasserorganismen, mit langfristiger Wirkung. |
| P273: | Freisetzung in die Umwelt vermeiden. |
| P280: | Schutzhandschuhe / Augenschutz / Gesichtsschutz tragen. |
| P305+P351+P338: | BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen. |
| Aussehen: | Pulver |
| Aggregatzustand: | fest |
| Lagerung: | 2 - 8 °C |
Zusammensetzung
| Makronährstoffe | |||
| Kaliumdihydrogenphosphat | KH2PO4 | 68,000 | mg/l |
| Calciumchlorid | CaCl2 | 166,000 | mg/l |
| Ammoniumnitrat | NH4NO3 | 720,000 | mg/l |
| Kaliumnitrat | KNO3 | 950,000 | mg/l |
| Magnesiumsulfat | MgSO4 | 90,270 | mg/l |
| Mikronährstoffe | |||
| Kupfer(II)Sulfat Pentahydrat | CuSO4· 5 H2O | 0,025 | mg/l |
| Eisenchelat | FeNaEDTA | 36,700 | mg/l |
| Borsäure | H3BO3 | 6,200 | mg/l |
| Mangan(II)Sulfat Monohydrat | MnSO4 · H2O | 18,940 | mg/l |
| Natriummolybdat Dihydrat | Na2MoO4· 2 H2O | 0,250 | mg/l |
| Zinksulfat Heptahydrat | ZnSO4· 7 H2O | 10,000 | mg/l |
| Vitamine | |||
| Biotin | 0,050 | mg/l | |
| Folsäure | 0,500 | mg/l | |
| Glycin | 2,000 | mg/l | |
| myo-Inositol | 100,000 | mg/l | |
| Nikotinsäure | 5,000 | mg/l | |
| Pyridoxin HCl | 0,500 | mg/l | |
| Thiamin HCl | 0,500 | mg/l | |
The newest safety datasheets or certificate of analysis (CoA) can get requested by using our contact form. They also can be requested during the order process or directly per mail via [email protected].
