The original composition of the NLN medium was described by Nitsch & Nitsch (1967). The medium was developed for the isolation of microspores from Brassica napus. The microspores were used to generate haploid plants (Lichter, 1982).
This product only contains the nutrient salts (macro & microelements), the organics (vitamins & amino acids) must be added separately after autoclaving.
References:
Nitsch, C & JP Nitsch. 1967. The induction of flowering in vitro in stem segments of Plumbago indica L. Planta 72(4): 355-370
Lichter, R. 1981. Anther culture of Brassica napus in a liquid culture medium. Zeitschrift für Pflanzenphysiologie 103(3): 229-237
Lichter, R. 1982. Induction of haploid plants from isolated pollen of Brassica napus. Zeitschrift für Pflanzenphysiologie 105(5): 427-434
| Media components: | macro elements micro elements |
| Release class: | Release class 0 |
| GHS-Pictograms: | 07 |
| GHS signal word: | Warning |
| Dosage: | 736 mg/l |
| Hazard Statements: | H315-H319-H335-H412 |
| Precautionary Statements: | P273-P280 |
| Storage Temperature: | 15 - 25 °C |
Achtung! |
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| H315: | Verursacht Hautreizungen |
| H319: | Verursacht schwere Augenreizung. |
| H335: | Kann die Atemwege reizen. |
| H412: | Schädlich für Wasserorganismen, mit langfristiger Wirkung. |
| P273: | Freisetzung in die Umwelt vermeiden. |
| P280: | Schutzhandschuhe / Augenschutz / Gesichtsschutz tragen. |
| P305+P351+P338: | BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen. |
| Aussehen: | Pulver |
| Aggregatzustand: | fest |
| Lagerung: | 2 - 8 °C |
Zusammensetzung
| Makronährstoffe | |||
| Calciumnitrat Tetrahydrat | Ca(NO3)2 · 4 H2O | 500,000 | mg/l |
| Kaliumdihydrogenphosphat | KH2PO4 | 125,000 | mg/l |
| Kaliumnitrat | KNO3 | 125,000 | mg/l |
| Magnesiumsulfat | MgSO4 | 61,000 | mg/l |
| Mikronährstoffe | |||
| Cobalt(II)Chlorid Hexahydrat | CoCl2 · 6 H2O | 0,025 | mg/l |
| Kupfer(II)Sulfat Pentahydrat | CuSO4 · 5 H2O | 0,025 | mg/l |
| Eisenchelat | FeNaEDTA | 36,700 | mg/l |
| Borsäure | H3BO3 | 10,000 | mg/l |
| Mangan(II)Sulfat Monohydrat | MnSO4 · H2O | 18,950 | mg/l |
| Natriummolybdat Dihydrat | Na2MoO4· 2 H2O | 0,250 | mg/l |
| Zinksulfat Heptahydrat | ZnSO4 · 7 H2O | 10,000 | mg/l |
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