NLN Amino Acid / Vitamin Premix

Item number: MD124

Category: Amino acid mixtures


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The original composition of the NLN medium was described by Nitsch & Nitsch (1967). In 1981 the composition was modified to a 13 % sucrose containing medium (NLN-13) by Lichter (1981). The medium was developed for the isolation of microspores from Brassica napus. The microspores were used to generate haploid plants (Lichter, 1982).
L-glutamine, L- serine and glutathione serves as sources for amino acids.


Nitsch, C & JP Nitsch. 1967. The induction of flowering in vitro in stem segments of Plumbago indica L. Planta 72(4): 355-370
Lichter, R. 1981. Anther culture of Brassica napus in a liquid culture medium. Zeitschrift für Pflanzenphysiologie 103(3): 229-237
Lichter, R. 1982. Induction of haploid plants from isolated pollen of Brassica napus. Zeitschrift für Pflanzenphysiologie 105(5): 427-434

Release class: Release class 0
GHS-Pictograms: 00
Dosage: 1.039 g/l
Storage Temperature: 2 - 8 °C

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