Cellulase Onozuka R-10

Item number: NC101

Category: Cellulase

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Cellulase, from Trichoderma viride. Multienzymatic system (Beldman et al. 1985) with Cellulase-, a-Amylase-, Hemicellulase-, Pectinase- and Protease-activity. It is used e.g. for protoplast isolation, often in combination with Macerozym (Potrykus & Shillito 1986).

The activity gets inhibited by Glucose, Cellobiose and Hg2+. Slightly inhibition occurs by Mn+, Ag2+, Zn2+ und Cu2+.

Stability of stock solutions: Stable for 24 h at 4 °C (pH 5-7); complete Degradation at 80 °C after 10-15 min.




References:

Beldman, G et al.. 1985. Eur. J. Biochem. 146: 301-308
Potrykus, J & RD Shillito. 1986. Methods Enzymol. 118: 549-578


GHS-Pictograms: 08
Synonyms: 1,4-(1,3:1,4)-ß-D-Glucan 4-glucano-hydrolase
CAS-Number: 9012-54-8
GHS signal word: Danger
EC-Number: 3.2.1.4
Hazard Statements: H334
EG-Number: 232-734-4
Hazard statements written: May cause allergy or asthma symptoms or breathing difficulties if inhaled
Precautionary Statements: P261; P342+P311
Solubility in water: 1 g/l (20 °C)
Precautionary statements written: Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray; If experiencing respiratory symptoms: Call a POISON CENTER/doctor
Storage Temperature: 2 - 8 °C

Gefahr!

GHS symbol 01 Health hazard
H334: Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen.
   
P261: Einatmen von Staub / Rauch / Gas / Nebel / Dampf / Aerosol vermeiden
P342+P311: Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM oder Arzt anrufen.
Cellulase: 10,00 U/mg
Hemicellulase: 1,00 U/mg
a-Amylase: 0,80 U/mg
Pectinase: 0,40 U/mg
Protease: 0,01 U/mg
   
Aussehen: Beiges Pulver
Aggregatzustand: Fest
Temperatur-Optimum: 50 - 60 °C
pH-Optimum: 4 - 5
Lagerung: 2 - 8 °C

Cellulase-Aktivität: 1 U katalysiert die Freisetzung von 1 µM Glucose aus Carboxymethylcellulose pro Minute bei 40 °C, pH 4.5.

Technische Daten

Löslichkeit in Wasser: 1 g/l (20 °C)
pH Wert (10%): 6 (20 °C)
CAS-Nr.: 9012-54-8
EC-Nr.: 232-734-4

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